RESUMO
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood (99.5%). The specificity of CATT on diluted blood was similar (99.3%). The highest sensitivities (100%) were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood.
Assuntos
Testes de Aglutinação/métodos , Ensaio de Imunoadsorção Enzimática/métodos , Testes de Fixação do Látex/métodos , Trypanosoma brucei gambiense/imunologia , Tripanossomíase Africana/diagnóstico , Testes de Aglutinação/economia , Testes de Aglutinação/normas , Animais , Anticorpos Antiprotozoários/sangue , Anticorpos Antiprotozoários/imunologia , Estudos de Casos e Controles , Líquido Cefalorraquidiano/parasitologia , Análise Custo-Benefício , Estudos Transversais , Doenças Endêmicas/estatística & dados numéricos , Ensaio de Imunoadsorção Enzimática/economia , Ensaio de Imunoadsorção Enzimática/normas , Hematócrito , Humanos , Testes de Fixação do Látex/economia , Testes de Fixação do Látex/normas , Linfa/parasitologia , Programas de Rastreamento , Parasitologia/economia , Parasitologia/métodos , Vigilância da População , Estudos Prospectivos , Sensibilidade e Especificidade , Sudão/epidemiologia , Tripanossomíase Africana/sangue , Tripanossomíase Africana/epidemiologia , Tripanossomíase Africana/imunologiaRESUMO
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis [CATT] on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood [99.5%]. The specificity of CATT on diluted blood was similar [99.3%]. The highest sensitivities [100%] were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood
Assuntos
Estudos Soroepidemiológicos , Sensibilidade e Especificidade , Inquéritos Epidemiológicos , Ensaio de Imunoadsorção Enzimática , Tripanossomíase AfricanaRESUMO
Cultures of insect forms of Trypanosoma congolense stocks and clones with different susceptibilities to trypanocidal drugs in vivo were initiated from bloodstream trypomastigotes harvested from mammalian hosts and maintained axenically in vitro at 27 degrees C. Growth inhibition of procyclic forms of susceptible and drug-resistant T. congolense occurred after incubation for 48 h with 0.1 ng isometamidium chloride ml or 500 ng diminazene aceturate/ml. Procyclic forms were propagated in vitro in the presence of 10 or 100 ng diminazene aceturate/ml for 20 days without or with only minor loss of growth. In the presence of 0.1 or 1 ng isometamidium chloride/ml the trypanocidal effect was detectable after 6-8 days and the cultures died after 10-12 days. Concentrations of 1 or 10 ng isometamidium chloride/ml had an irreversible effect when the trypanosomes were exposed to the drug for 24 h. Both drugs used in this study thus showed antitrypanosomal activity on T. congolense procyclic forms but it was not possible to distinguish between drug-resistant and susceptible stocks or clones employing the criteria of growth inhibition or death of trypanosomes in culture.
